Novel Protein Kinase Signaling Systems Regulating Lifespan Identified by Small Molecule Library Screening Using Drosophila
Figure 2
Effects of the protein kinase inhibitors on intracellular signaling in Drosophila S2 cells treated for 48 hours with the protein kinase inhibitors indicated at the bottom of the panels.
Panel A shows data obtained using antibodies to phospho-Erk1/2 (white bars), and total Erk1/2 (black bars). Panel B shows data obtained using antibodies to phospho-Mek1/2 (white bars), and total Mek1/2 (black bars). Panel C shows data obtained using antibodies to phospho-p38 MAPK (white bars), and total p38 MAPK (black bars). Panel D shows data obtained using antibodies to phospho-JNK (white bars), and total JNK (black bars). The height of each bar represents the the signal normalized to the total amount of protein in each sample as judged by comparison to several apparently invariant protein bands observed on the blot by Ponceau S staining (representative staining is shown in Figure S4A). One asterisk indicates the changes were significant (P≤0.05); two asterisks indicates the results were highly significant (P≤0.01), and three indicates it is very highly significant (P≤0.001). See Figure S4A for representative Western blots and protein bands visualized using Ponceau S staining of membranes.