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Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies

Figure 5

Fluorescent eye markers allow visual identification of double and triple transgenic mice.

(A) Double transgenic mice carrying the EGFP marker and either tdTomato or mPlum can be easily distinguished from single transgenic littermates under the GFP flashlight. (B) Single, double and triple transgenic mice can be identified using mCFP, mOrange and mPlum eye markers. All six possible combinations are distinguishable under the GFP flashlight. Insets show close up view of fluorescence in the eyes of double and triple transgenic mice.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0029486.g005