Polycomb Repressive Complex 2 Targets Murine Cytomegalovirus Chromatin for Modification and Associates with Viral Replication Centers
Figure 2
Measurement of H3K27me3 enrichment at the MIE locus of MCMV.
(A) Graphical illustration of the MIE locus of MCMV. Solid black boxes represent regions probed for H3K27me3 & H3K4me3 enrichment. Regions denoted in figure: (1) Represents the Enhancer 2 region, (2) the transcriptional start site (TSS) of the IE1-3 transcriptional unit and (3) represents Exon 1 within the ORF of the IE1-3 locus. +1 transcriptional start sites are indicated with black arrows. Enhancer regions are marked in hatched boxes. IE1-3 exons are depicted as gray arrows. (B) H3K27me3 is enriched at the MIE locus at pre-IE times during MCMV infection. ChIPs using anti-H3K27me3 antibody were analyzed by Q-PCR using primer/probe sets specific for the indicated loci at 1.5, 3, 6 and 12 hpi as indicated. Each graph displays the mean value and S.E.M. for each queried locus, with data from three independent ChIPs. The results are presented as the IgG subtracted %Input of the queried locus normalized to the IgG subtracted β-Actin %Input. Samples with values that vary significantly from the negative control, Dlx1,are indicated by asterisks (* P value<0.05, ** P value<0.01; ANOVA).