Functional Induction of the Cystine-Glutamate Exchanger System Xc- Activity in SH-SY5Y Cells by Unconjugated Bilirubin
Figure 5
Response to H2O2 oxidative stress of SH-SY5Y cells pretreated with bilirubin and GSH intracellular levels of the cells before H2O2 treatment.
SH-SY5Y cells were exposed to two successive treatments: the first with 140 nM Bf (control with 0.6% DMSO medium) for 24 h. After this treatment, cells were released in growth medium for 156 h. Immediately after exposure to UCB (column A) and 156 h (column B) of release, cells were incubated, as second treatment, with different concentrations of H2O2 for 60 min and viability evaluated by MTT. At each time viability is expressed as a percentage of the controls considered as 100%. At each time viability results are compared with GSH intracellular levels of the cells before H2O2 treatment. Results are expressed as media ± SD of four different experiments.