Inducible Genetic Lineage Tracing of Cortical Hem Derived Cajal-Retzius Cells Reveals Novel Properties
Figure 2
The progenitor zone that will later generate CR cells is specified as early as E6.5.
A–B: Whole-mount X-gal staining of E18.5 Fzd10 CreER™/R26R-LacZ brains when TM induction was given at E6.5. Arrows show CR cells migrating out of the cortical hem. C–H: Double immunostaining shows that induced YFP-positive cells are reelin positive at E16.5 (C–E, arrows) and E18.5 (F–H, arrows) in the neocortical MZ. I–N: Double immunostaining shows that induced YFP-positive cells are also P73 positive. O–Q: YFP and P73 are co-localized at the marginal zone of the hippocampal dentate gyrus (arrows). DG, dentate gyrus; MZ, marginal zone. Scale bars:A, B: 1 mm; C–E: 225 µm; others: 200 µm.