De Novo Growth Zone Formation from Fission Yeast Spheroplasts
Figure 2
The Cdc42 pathway affects the width of growth zones formed de novo.
A. The width of a spheroplast growth zone is the same as the width of a non-spheroplasted cell, but is altered by the mutation of genes that control cell morphology. Cell widths are represented in box-and-whiskers plots. All widths except for the first point are from late recovery spheroplasts, measured as indicated in the cartoon, 7–8 h after spheroplast formation; 50 cells were measured for each genotype or treatment. B. Late recovery spheroplasts of the indicated genotypes. DIC microscopy. C. Addition of 50 µM MBC depolymerizes microtubules, leaving only short stubs, but does not inhibit spheroplast growth zone formation. Top row: Atb2-GFP marks microtubules, and is shown as a maximum projection of Z-series that spanned the cell width, LUTs inverted. Bottom row: Cell shape by DIC. D. Spore germination forms a de novo growth zone, similar to spheroplast recovery. Right column shows growth zone widths, 50 cells measured for each genotype, displayed ±S.D. Wild-type exponentially growing cell width = 3.88 µm±0.15; scd2Δ exponentially growing cell width = 5.34 µm±0.25. DIC microscopy. All scale bars represent 5 µm.