Oxidation of DJ-1 Induced by 6-Hydroxydopamine Decreasing Intracellular Glutathione
Figure 5
Cytotoxicity and DJ-1 oxidation induced by electrophilic benzoquinone (BzQ).
(A) Cells were treated with BzQ at various concentrations for 20 h and subjected to the MTT assay. ** Significantly different from the value of 0 µM (n = 3, p<0.01, Tukey, ANOVA). (B) Cell samples obtained from the SH-SY5Y cells treated with the indicated conditions for 3 h were separated by 2D-PAGE and subsequently subjected to western blot analysis using the anti-DJ-1 antibody. The ratio of oxidized DJ-1 is shown under each condition (n = 2).