Ribavirin Enhances IFN-α Signalling and MxA Expression: A Novel Immune Modulation Mechanism during Treatment of HCV
Figure 3
MxA protein expression is enhanced with the addition of IFN-α and Ribavirin.
(A) Immunoblot of lysates immunoprecipitated for MxA and probed for MxA from Huh7s treated with IFN-α for 2 h, Ribavirin for 2 h or both for 2 h (n = 3). (B) Confocal micrograph of Huh7s treated with IFN-α for 2 h, Ribavirin for 2 h or both for 2 h (n = 3). Confocal micrograph shows MxA and the nucleus, labelled with Alexa 488 and DAPI, respectively. Bar, 10 µm.