Evolution of the Bovine TLR Gene Family and Member Associations with Mycobacterium avium Subspecies paratuberculosis Infection
Figure 1
For validated bovine TLR SNPs detected via pyrosequencing (n = 244), a regression analysis was performed for pyrosequencing allele frequency (AF) estimates corresponding to the true minor alleles.
(<0.5), as defined by allele-specific genotyping assays, and minor AFs (MAFs) directly ascertained by genotyping (n = 96 elite sires; 31 breeds). The true minor alleles (<0.5) were correctly identified for 236/244 (97%) SNPs via pyrosequencing. This analysis provided strong statistical evidence (P = 0.999846; Ho: slope = 1) for little or no bias in the pyrosequencing-based estimates of allele frequency.