Recruitment of Oct4 Protein to UV-Damaged Chromatin in Embryonic Stem Cells
Figure 3
ATP depletion and Oct4 levels at UV-damaged chromatin.
A, In comparison with control (a), ATP depletion reduced the diameter of mitochondria (b). c, Panel shows the comparison of the average diameter of mitochondria in control cells and ATP-depleted cells. d, Mitochondria staining in control cells. e, Mitochondria staining after ATP depletion. B, In comparison with control nonirradiated cells (a), GFP-Oct4 become focally distributed after ATP depletion (b). C, DNA lesions were induced in GOWT1 mESCs after ATP depletion, but the absence of GFP-Oct4 accumulation was observed. γH2AX positivity was low and homogeneous within entire cells. D, H3K9 acetylation levels were low in UV-irradiated chromatin after ATP depletion. Scale bars: in each panel, the scale bar is shown with the relevant value.