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Protein Characterization of a Candidate Mechanism SNP for Crohn's Disease: The Macrophage Stimulating Protein R689C Substitution

Figure 4

SPR competition assays.

(A–D) Bindings to immobilized RON-Sema-PSI-IPT4 of mixtures containing MSPβ with varying concentrations of RON-Sema or RON-Sema-PSI-IPT4. (E–H) The dependence of free MSPβ concentrations on RON concentration in the solution mixtures. The free MSPβ concentrations were determined from the measured relative response based on a calibration curve.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0027269.g004