Calcium-Release Channels in Paramecium. Genomic Expansion, Differential Positioning and Partial Transcriptional Elimination
Figure 12
CRC-VI-3 localize to early endosomes.
Double labeling of cells using antibodies against CRC-VI-3 (green) and α-tubulin (red). CRC-VI-3 specific antibodies recognize a punctate pattern at the cell cortex in close proximity to, but slightly below the basal bodies which were stained with α-tubulin antibodies (compare z1 slices shown in A and A′ with z2 slices in B and B′; for merge see B″). The positioning of the CRC-VI-3 labeled organelles below basal bodies (arrows) is also shown in median sections (C, C′, C″) as well as in cross-sections of the oral apparatus (oa; D, D′, D″). All this demonstrates localization of CRC-VI-3 to early endosomes. Moreover, in a dorsal view of the cell CRC-VI-3 is present at the pores (po) of both contractile vacuole complexes (E, E′, E″). Bars = 10 µm.