Carbon Dynamics, Development and Stress Responses in Arabidopsis: Involvement of the APL4 Subunit of ADP-Glucose Pyrophosphorylase (Starch Synthesis)
Figure 3
Characterization and effect on gene expression of the T-DNA insertion in the eat1 Arabidopsis mutant.
T-DNA insertion was localised between the At2g21580 and At2g21590 genes (black bars), which encode, respectively, the 40S ribosomal protein S25, and an ADP-glucose pyrophosphorylase large subunit, recently characterized as APL4 [21] (A). The grey bar at the 5′ end of insertion represents a 21 bp unknown sequence and the flag corresponds to the BH755830 line [52]. The positions of the At2g21580 and At2g21590 genes, of the mutant line and of the T-DNA, on the F2G1 BAC clone are given. Expression of At2g21590 gene in plantlets cultivated on MS-agar medium and on soil was analysed by real-time RT-PCR (B). Total RNA was isolated from 30-day-old plantlets grown on 1x MS-agar medium and from 5-week-old plantlets grown for 15 days on 1x MS-agar medium, transferred to soil and further grown under controlled conditions (16-h light at 22°C and 8-h dark at 18°C). The Ws ecotype was used as WT. At2g21590 mRNA levels were normalized with respect to housekeeping genes ubiquitin5 and β–tubulin. Values are the mean (± S.E.M.) of six measurements. Asterisks represent statistically significant differences (Mann-Whitney test, P<0.05) between WT and eat1. These experiments were carried out twice and results were similar.