The Adaptor Protein SH2B3 (Lnk) Negatively Regulates Neurite Outgrowth of PC12 Cells and Cortical Neurons
Figure 8
SH2B3 inhibits neurite extension of cortical neurons.
(A) Isolated E18 rat cortical neurons were transfected with GFP, GFP-SH2B3, GFP-SH2B3(1–296), or GFP-SH2B3(296–575) on DIV 4. Images were taken on DIV 5. Scale bar: 50 µm. (B) E18 rat cortical neurons were treated as in (A). Transfected neurons were shown on the upper panels. Cells were subjected to immunofluorescence staining using anti-Tau-1 antibody and Alexa Fluor 555-conjugated secondary antibody (lower panels). Tau-1 is an axonal marker. Arrow points to GFP-SH2B3(296-575)-transfected cell. Images were taken using Zeiss Observer Z1 microscope. Scale bar: 50 µm. (C) E18 rat cortical neurons were treated as in (A). The averaged neurite length on DIV 5 was measured using Image J software. Total numbers of neurons analyzed are shown on the bars. *: P<0.05, one-way ANOVA.