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dTip60 HAT Activity Controls Synaptic Bouton Expansion at the Drosophila Neuromuscular Junction

Figure 3

Postsynaptic reduction of dTip60 in the nervous system leads to a decrease of synaptic boutons at larval NMJs.

Flies homozygous for either dTip60E431Q, dTip60RNAi or control w1118 were crossed to flies homozygous for the muscle specific GAL4 driver MEF-2, and staged third instar progeny larvae were collected. Confocal imaging analysis of larval boutons on muscles 6/7 at abdominal segment A4 double labeled with anti-HRP (green) that labels the entire presynaptic membrane and anti-dTip60 antibody (blue). (A and B) control w1118 larvae (C and D) larvae expressing dTip60E431Q (E and F) larvae expressing dTip60RNAi. Scale bar 10 um. Line arrow depicts Ib bouton, thick arrow depicts Is bouton. Histogram depicts quantitative analysis of bouton number on muscles 6 and 7 at abdominal segment 4 where (G) represents total bouton number (H) number of Is boutons. In the analyses, w1118 genotype is represented by 18 larval preparations (n = 18), dTip60RNAi (n = 19) and dTip60E431Q (n = 19). Asterisks (*) indicates statistically significant difference in relation to control where single asterisks indicate p≤0.001 and double asterisks indicate p≤0.0001. All error bars depict standard error of the mean.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0026202.g003