Aggravation of Chronic Stress Effects on Hippocampal Neurogenesis and Spatial Memory in LPA1 Receptor Knockout Mice
Figure 5
Number and maturation of DCX+ neurons.
Mean values ± SEM. (A) The total number of young, DCX+ neurons was reduced in the absence of the LPA1 receptor. Chronic stress decreased the DCX+ population only in the mutant genotype. (B) Chronic stress increased the proportion of DCX+ cells that remained in the more immature stages of development (A–D cells, arrows) in mice lacking the LPA1 receptor. (C) Moreover, the dendritic arborisation in the more mature DCX+ cells (E–F) was less developed in stressed null mice. A representative drawing is shown next to the graph. (- %): reduction from the control group. Scale bar in A: 100 µm; B: 20 µm. Post-hoc LSD tests: (*p<.05; **p<.001): significant difference between the stressed group vs. its control; (#p<.05): significant difference between the control NULL vs. the control WT mice.