Distinct Populations of Hepatic Stellate Cells in the Mouse Liver Have Different Capacities for Retinoid and Lipid Storage
Figure 3
Total retinyl ester and triglyceride levels in liver and HSCs.
(A) Retinyl ester (RE) levels in livers from WT and collagen-GFP mice, expressed as µg RE per gram liver weight. (B) Retinyl ester levels in AF-HSCs and GFP-HSCs, expressed as µg RE per million cells. (C) Triglyceride (TG) levels in livers from WT and collagen-GFP mice, expressed as µg TG per gram liver weight. Mice were fasted for 4 hours prior to sacrifice and tissue collection. (D) TG levels in AF-HSCs and GFP-HSCs, expressed as µg TG per million cells. Significance was determined by a Student's t-test, * p-value<0.05. (E) Protein levels of LRAT in AF-HSCs and GFP-HSCs. Experiment was repeated with similar results.