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Destruction of Lymphoid Organ Architecture and Hepatitis Caused by CD4+ T Cells

Figure 3

Immunofluorescence analysis of B and T cell regions.

(A) BL/6 mice, BL/6 mice depleted of CD8+ T cells, and BL/6 mice depleted of CD8+ T cells receiving 1.5×107 purified LCMV-immune CD4+ T cells were infected with LCMV. On day 11 after infection spleen sections were stained for CD3 (T cells; green), B220 (B cells; red), Laminin (blue), or CD35 (FDCs; green), gp38 (FRCs; red), Desmin (stromal cells, blue), or BP-3 (follicular stromal cells; red) and Desmin (green). One representative section of 3 mice per group is shown. BL/6 naïve mice served as control. Size bar represents 100 µm. The top row provides an overview with the rows below showing a higher magnification and consecutive sections. (B) The same experimental groups as in (A) were analyzed for the expression of CXCL13, CCL21, and CCL19 in the spleen by rtPCR. Each sample was analyzed in duplicate. Each symbol represents the mean of one animal. Horizontal bars indicate mean.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0024772.g003