Structure of Apo- and Monometalated Forms of NDM-1—A Highly Potent Carbapenem-Hydrolyzing Metallo-β-Lactamase
Figure 1
NDM-1 structure and comparisons with selected carbapenemases.
A. Aerial view of active site (represented by two phosphate ions), the protein is made up of four layers, α/β/β/α, and forms a sandwich. Secondary structure elements and N- and C-termini are labeled. B. Comparison of K. pneumoniae NDM-1 (PDB id 3RKJ – magenta, N and C-termini are labeled) with three MBLs with carbapenemase activity P. aeruginosa VIM-2 (PDB id 2YZ3 - wheat), P. aeruginosa IMP-1 (PDB id 1DD6 - light blue) and P. aeruginosa VIM-4 (PDB id 2WHG - pale green). Loops contributing to the active site are labeled ASL1–5. Zn1 and Zn2 (blue spheres) are from the structure of VIM-2.