The Functioning of the Drosophila CPEB Protein Orb Is Regulated by Phosphorylation and Requires Casein Kinase 2 Activity
Figure 3
ck2 is needed for grk signaling.
Frequency of eggs with ventralized chorions produced by ck2α, ck2β and various ck2/orb trans-heterozygotes. Unless otherwise indicated all trans combinations are heterozygous for the mutant allele. Genotypes are: orb/+: HD19G orbF343/+ (n = 1808); Tik/+: ck2αTik/+ (n = 1788, p-value = 0.00055); Tik/orb: ck2αTik/HD19G orbF343 (n = 1554: p-value = 2.01E-45); TikR/+: ck2αTikR/+ (n = 1295: p-value = 3.51E-59); TikR/orb: ck2αTikR/HD19G orbF343 (n = 2233: p-value = 0.46515); orb/+: HD19G orbF343/+ females (n = 1467); mbu/+: ck2βmbuΔA26-2L/+ (n = 1505: p-value = 5.83E-61); mbu/orb: ck2βmbuΔA26-2L/HD19G orbF343 (n = 1892: p-value = 4.91E-85); And/And: ck2βAnd/ck2βAnd (n = 1450: p-value = 3.62E-29); And/orb: ck2βAnd/HD19G orbF343 (n = 1577: p-value = 0.000000001); Tik/mbu: ck2αTik/ck2βmbuΔA26-2L (n = 1034). For wild type females, less than one percent of the eggs have ventralized chorions.