Haemolysis during Sample Preparation Alters microRNA Content of Plasma
Figure 5
Levels of potential biomarkers for CAD in haemolysed and non-haemolysed plasma.
Levels of miR-92a and 155 were measured in matching haemolysed and non-haemolysed plasma samples from different EDTA tubes of the same blood collection from three different MM or CAD patients and 1 healthy control. (A) The increases in miRNA levels in haemolysed plasma as compared to non-haemolysed plasma, without normalisation to miR-16 calculated using 2−ΔCq. (B) Changes in miR-92a and miR-155 levels in haemolysed plasma when calculated using the 2−ΔΔCq method with miR-16 as reference. MM1, MM2, CAD1, N1 = Pairs of haemolysed and non-haemolysed plasma from four patients/controls.