Excessive Food Intake, Obesity and Inflammation Process in Zucker fa/fa Rat Pancreatic Islets
Figure 1
Determination of plasma cytokines levels in fa/+ and fa/fa rats.
A) Chemiarray: Rat cytokine array membranes were incubated with 1 ml of 2-fold diluted fa+ or fa/fa rats plasma for 2 h. After removing unbound materials, membranes were incubated with a mixture of biotin-labeled antibodies and controls with TBS buffer only. Signals were detected with HRP-conjugated streptavidin and ECL. The relative expression levels of cytokines were determined by densitometry and plotted from 4 fa/+ and 4 fa/fa rats. The array membranes were scanned with a chemiluminescence imager. Signal densities were normalized and background corrected. B) Bio-Plex rat cytokine analysis. Cytokines concentrations were calculated automatically with Bio-Plex Manager software, using a standard curve derived from a recombinant cytokine standard. Concentrations are given in picograms per milliliter.