The UNC-45 Chaperone Is Critical for Establishing Myosin-Based Myofibrillar Organization and Cardiac Contractility in the Drosophila Heart Model
Figure 5
M-Mode analysis and quantification of heart rate in unc-45 KD and control third instar larvae, young pupae and adult hearts.
Cardiac parameters were determined as described in Materials and Methods. (A, B) M-modes (4 sec) from control (top) and unc-45 KD (bottom) third instar larvae and young pupae (as described in Figure 1). (C) Mean heart rate of unc-45 KD in third instar larvae and young pupae were significantly increased (tachycardia) compared to control. In contrast, heart rates were significantly reduced in adult hearts compared to controls. Heart rate data are shown as means ± SD; statistical significance was determined using a multivariate Student's t test (** = p<0.01 and *** = p<0.001).