Control of Gene Expression by the Retinoic Acid-Related Orphan Receptor Alpha in HepG2 Human Hepatoma Cells
Figure 1
qRT-PCR analysis of expression of several of the genes found to be up-regulated in HepG2 cells over-expressing RORα upon micro-array analysis.
Aliquots of the RNA samples from stably-transfected HepG2 cells over-expressing RORα1 and from stably transfected control cells that have been analyzed by the DNA micro-array technology were submitted to qRT-PCR analysis for measuring the levels of Rora, SPARC, PLG, G6PC, NR1D2, and AGRP mRNAs. The mRNA levels were normalized to the 36B4 mRNA level. Results corresponding to HepG2 cells over-expressing RORα1 (black bars) are given relatively to the control cells (grey bars). Values refer to the means +/− S.E.M. of the fold changes (n = 3 independent pools of transfected cells). *: P<0.05, **: P<0.01, ***: P<0.001 between RORα1 over-expressing and control cells.