Functional Hair Cell Mechanotransducer Channels Are Required for Aminoglycoside Ototoxicity
Figure 3
Open MET channels are required for GTTR entry into hair cells.
A. Following incubation in control media for 24 hr, P3 rat cochleae treated with GTTR alone (3 µM for 1 hr at 37°C) showed robust uptake in inner (asterisk) and outer hair cells (OHC) in the middle and basal cochlear turns. B. Co-treatment with curare (1 mM) effectively prevented GTTR uptake. C–D. Quantification of GTTR fluorescent intensity demonstrated that uptake is significantly reduced by blocking MET channels with curare in both the inner and outer hair cells throughout the cochlear turns. Error bars = S.D. Scale bars = 100 µm.