Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Characterization of Cortical Neuronal and Glial Alterations during Culture of Organotypic Whole Brain Slices from Neonatal and Mature Mice

Figure 3

Immunohistochemical labeling of cytoskeletal proteins in cultured brain slices.

Organotypic brain slice cultures from P6 (A), 25–28(B) and 50+(C) day old mice, cryogenically subsectioned at 40 µm. Slices were cultured for a period of 7DIV before immunohistochemical analysis with neuronal cytoskeletal markers NF-L and SMI312 in addition with a nucleus dye (Nuclear yellow). Nuclear yellow staining is pseudo-coloured blue. Scale bar 100 µm.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0022040.g003