Characterization of Cortical Neuronal and Glial Alterations during Culture of Organotypic Whole Brain Slices from Neonatal and Mature Mice
Figure 3
Immunohistochemical labeling of cytoskeletal proteins in cultured brain slices.
Organotypic brain slice cultures from P6 (A), 25–28(B) and 50+(C) day old mice, cryogenically subsectioned at 40 µm. Slices were cultured for a period of 7DIV before immunohistochemical analysis with neuronal cytoskeletal markers NF-L and SMI312 in addition with a nucleus dye (Nuclear yellow). Nuclear yellow staining is pseudo-coloured blue. Scale bar 100 µm.