Horizontal Transmission of Candida albicans and Evidence of a Vaccine Response in Mice Colonized with the Fungus
Figure 5
C. albicans colonization of mice may result in appearance of serum antibodies specific for a mannan extract of the fungus.
By day 71, serum antibody was especially obvious in mice that were treated with antimicrobial agents and received C. albicans in their drinking water (i.e., groups A, C and G;see Table 3). Only one mouse from each of two groups that became colonized later as a result of horizontal transmission had evidence of serum antibody (groups B and D). Note that the sera from groups H and H' were not properly marked for identification of H or H' and were, thus, both designated as H* and H*. The positive control (+ctrl) for the ELISA was monoclonal antibody B6.1, which is specific for a mannan component in the C. albicans cell wall. The negative control (-ctrl) contained all of the ELISA reagents except for primary antibody, which was omitted.