Hydrogen Peroxide Acts on Sensitive Mitochondrial Proteins to Induce Death of a Fungal Pathogen Revealed by Proteomic Analysis
Figure 2
Proteomic analysis of total cellular proteins indicated that mitochondria might be sensitive to H2O2.
(A) Differential expression of total cellular proteins in response to H2O2 in P. expansum visualized by 2D gel electrophoresis. The fungal spores were cultured in potato dextrose broth medium till germination, and then treated with 0 mM (control) or 30 mM H2O2 for 60 min at 25°C. Total cellular proteins were extracted from the fungi using a phenol-based method as described under “Materials and Methods”. Proteins (500 µg) were separated on 13 cm Immobiline Drystrip with a 4–7 linear pH gradient in the first dimension and visualized by Coomassie Blue staining. Numbers indicate proteins that were differentially expressed under H2O2 stress and subsequently identified by mass spectrometry (listed in Table S1). (B) Close-up views of putative mitochondrial proteins showing differential expression between control and H2O2-treated samples. (C) H2O2 induced collapse of mitochondrial membrane potential in the fungal pathogen as indicated by Mitotracker orange staining. The scale bar represents 20 µm.