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The Relative Importance of Topography and RGD Ligand Density for Endothelial Cell Adhesion

Figure 3

The number and cell area of endothelial cells is different on flat silicon and etched silicon.

Serum-starved endothelial cells were adhered for 30 min on chemically unmodified silicon that were etched for 0–90 min. (A) The number of adherent cells per square micron is significantly different between flat (0 min) and pyramid-presenting surfaces (10–90 min) as indicated by the asterisks (p<0.05). No difference was detected on etched surfaces (p>0.05). (B) Cell area was similar on all surfaces (p>0.05). Data correspond to a minimum of three independent experiments. Error bars represent standard deviations.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0021869.g003