Selective BRAFV600E Inhibitor PLX4720, Requires TRAIL Assistance to Overcome Oncogenic PIK3CA Resistance
Figure 7
PLX4720 requires TRAIL synergy to induce an anti-proliferative effect.
(A) Combined treatment with 1 µM PLX4720 for 8 hours and concomitant administration of a low (10 ng/ml) or higher (50 ng/ml) concetration of TRAIL for 16 hours in Caco-BR cells. The values are the average of three independent experiments and are presented as fold change of the absorbance of treated/ untreated cells, for each condition. The cytotoxic effects of TRAIL were assayed by SRB 24 hours after treatment. *P<0.05, **P<0.01, ***P<0.001. (B) Caspase-3 activation analysed by immunofluorescence 24 hours after induction with TRAIL/ PLX4720 alone or in combination, in a 3D culture of Caco-BR13 cells. Representative confocal immunofluorescence images, double labelled with Hoechst (blue) and active caspase-3 (green). Original magnification 63x.