Phenotypic Expression of ADAMTS13 in Glomerular Endothelial Cells
Figure 3
ADAMTS13 mRNA expression in cultured endothelial cells.
ADAMTS13 gene transcripts in endothelial cells were detected by real-time PCR. A. Using the probe against exons 28–29 the amplification plot indicates detectable ADAMTS13 mRNA levels in CiGEnC and HMVEC cells. Human kidney (HK) and human liver (HL) were used as the positive controls. No template control (NTC) and CHO cells were used as the negative controls and showed no amplification. B. The housekeeping gene 18S, which was expressed at comparable levels in all samples, was used to standardize the data. The upper limit of the boxes depicts the means and bars are standard error of the mean from three separate cell culture experiments.