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Synchrotron Radiation Micro-CT at the Micrometer Scale for the Analysis of the Three-Dimensional Morphology of Microcracks in Human Trabecular Bone

Figure 1

Diagram of the imaging process.

A: specimen (photograph and diagram) and specimen stage. Specimens are 5×5×10 mm and the imaged field of view is 2.8×2.8×2.2 mm. B: 2000 radiographs of the specimen are taken from evenly distributed angles from 0° to 180°. C : after tomographic reconstruction, the 3D image is obtained, composed of a stack of slices. D : 256×256 ROI of a reconstructed grey-level image(top), customized non-linear filtering (middle), and segmentation (bottom, microcracks in color, lacunae in white). E: 3D renderings can then be produced to observe and measure microarchitecture or microcracks.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0021297.g001