Food for Song: Expression of C-Fos and ZENK in the Zebra Finch Song Nuclei during Food Aversion Learning
Figure 3
Fluorescent immunostaining of IEG expression in HVC and RA after food aversion learning (A,C) or after singing (B,D).
C-Fos (red nuclear label) was expressed in HVC and RA after both behaviors, whereas ZENK (green nuclear label) only in the song group (colocalized expression of both IEGs, yellow, B, or white in the insert of B, and in D); neuron-specific NeuN labeling is shown in blue cytoplasmatic and nuclear label. Transverse sections. Scale bars, 0.2 mm (A and B), 0.1 mm (C and D).