Identification of the Allosteric Regulatory Site of Insulysin
Figure 3
Effects of distal site mutations.
(A) Comparison of the kinetics of wild type IDE with the distal site mutants. Activity was measured with varying amounts of Abz-GGFLRKHGQ-EDDnp substrate as indicated. Either hyperbolic (Michaelis-Menten equation) or sigmoidal curves (Hill equation) were fit to the data. (B) Effect of bradykinin on the activity of wild type IDE and the distal site mutants. (C) Activation of wild type IDE and distal site mutants by ATP. Relative activity is the fold activation above the level of the wild type enzyme alone.