Increased Systemic Glucose Tolerance with Increased Muscle Glucose Uptake in Transgenic Mice Overexpressing RXRγ in Skeletal Muscle
Figure 2
Levels of Glut 1 and Glut4, and glucose uptake in the skeletal muscle of RXRγ mice.
(A) Gene expressions of RXRγ, Glut1, and 4 were examined by quantitative real-time PCR. The value for wild-type (littermates of line 4-3) mice was set at 100, and relative values are shown. (B) Protein levels of Glut1 and Glut4 were examined by Western blotting. Results of relative densitometric signal for Glut1 and 4 are shown. (C) Glucose uptake in the absence or presence of insulin and (D) glycogen content were increased in the skeletal muscle of RXRγ mice. Ratio of enhanced glucose uptake in the presence of insulin (insulin/basal) was similar in control and RXRγ mice. In A, B and D, the same samples were used. Mice were males of 12 weeks of age. The number of animals was 6 for both control (open bars) and RXRγ (filled bars) mice. These samples were also used in Table 1. In C, mice were males of 24–27 weeks of age. The number of animals was 6 for both control (open bars) and RXRγ (filled bars) mice. * P<0.05 and ** P<0.01 compared with respective control. N. S., not significant.