In Vitro and In Vivo Germ Line Potential of Stem Cells Derived from Newborn Mouse Skin
Figure 6
Comparison of germ cell marker expression in OLCs from the stem cell (SC) group with that of oocytes (Ov) after culture for 12 days.
A. Real-time RT-PCR comparing the expression levels of Sox2, Nanog, Fragilis, and Stella. Data are mean ± SE of 3 experiments. (data were analysed using a t-test); * indicates P<0.05. B. RT-PCR showing the presence of Zp3 mRNA but the absence of Zp1 and Zp2 mRNA in OLCs (SC group) after 12 days of differentiation. C. Expression (red) of DAZL, STELLA, and VASA in OLCs (SC group) using newborn ovarian oocytes (Ov group) cultured under the same conditions as the positive control. Blue shows the staining of the nucleus. Scale bars: C, 25 µm.