Long-Lasting Inhibitory Effects of Fetal Liver Mesenchymal Stem Cells on T-Lymphocyte Proliferation
Figure 1
Characterization proliferation potential and immunosuppressive functions of adult and fetal MSC.
(a) Bone marrow MSC (n = 6), cultured between passage 2 and 4, and human fetal-liver MSC, cultured between passage 12 and 18 (n = 5), were characterized by cell surface-marker expression using flow cytometry. The gray curves indicate the corresponding negative mouse IgG1 or IgG2a antibodies. Data corresponds to a typical staining of BM-MSC and FL-MSC. (b) Time course of cell growth in culture of BM-MSC and FL-MSC. 5×104 of both MSCs were seeded in 6 wells plate (4×103/cm2). (c) Cell count of MSC after 6 days. Averages of three independent experiments are shown. (d) Activated lymphocytes (stimulated with anti CD3 and anti CD28) were cultured in the presence of adult MSC (passage 2–4, n = 3) or fetal MSC (passage 12–15, n = 3) at different T∶MSC ratios (CFSE assay, 5 µM for 5 min at 37°C). Averages of six independent experiments are shown.