T Cells from Programmed Death-1 Deficient Mice Respond Poorly to Mycobacterium tuberculosis Infection
Figure 7
PD-1−/− mice express reduced markers of autophagy after M. tb infection.
Macrophages were isolated from the peritoneum of WT and PD-1−/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.