High-Level Fluorescence Labeling of Gram-Positive Pathogens
Figure 5
EGFP fluorescence in bacterial strains harboring pBSU101 following overnight growth in the presence (+AB) or absence (−AB) of antibiotic pressure was determined by FACS analysis.
Fluorescence values obtained for each bacterial species following overnight growth under antibiotic pressure were set to a value of 1. Measurements were carried out for S. agalactiae, S. pyogenes, S. dysgalactiae subsp. equsimilis (SDSE) serogroup C, SDSE serogroup G, E. faecalis, E. faecium, S. mutans, S. anginosus, and Staphylococcus aureus. Shown are the results of three independent experiments and standard deviations.