Nrt1 and Tna1-Independent Export of NAD+ Precursor Vitamins Promotes NAD+ Homeostasis and Allows Engineering of Vitamin Production
Figure 7
Purification of NR from PAB076-Conditioned Media.
A) HPLC trace of media collected from strain PAB076 grown to OD 60 in 2x YPD and supplemented with 5 mM NA. B) Preparative SP-Sephadex chromatography with fractions analyzed by HPLC. NR eluted at 20 to 50 mM NaCl in fractions 27 to 36. C) Intracellular NAD+ determination of strain BY4742 grown in NA-free SDC media and NA-free SDC media supplemented with 10 µM NR from fractions produced in panel B.