Control of Glucosylceramide Production and Morphogenesis by the Bar1 Ceramide Synthase in Fusarium graminearum
Figure 7
Deletion of BAR1 causes disruption in hyphal tip organization.
A. Representative images of sterol-rich domains stained with filipin at the extreme apex of hyphae. Note the bright regions stained at the tips of wt and complemented strains (arrows) but missing from ΔBAR1 hyphae. All micrographs in panel A at the same scale. B. Endomembrane systems stained with FM4-64. Wild-type germlings exhibited bright spots at the extreme hyphal apex coinciding with the vesicle-rich Spitzenkörper (arrows). Staining at the hyphal tip of ΔBAR1 germlings failed to display a discrete Spitzenkörper. All micrographs in panel B at the same scale. Scale bars = 10 µm.