Amyloid Beta Resistance in Nerve Cell Lines Is Mediated by the Warburg Effect
Figure 4
Chemically inhibiting LDHA or PDK1 decreases cell viability in Aβ resistant cells.
A significant decrease in cell viability in both PC12 (A) and B12 (B) resistant lines was observed after 48 hr concomitant exposure to Aβ (20 µM) and 20 mM oxamate (ox), a chemical inhibitor of LDHA (*P<0.01). Similarly, 48 hr Aβ exposure significantly decreased cell viability in both PC12 (C) and B12 (D) resistant lines when cells were co-treated with 2.5 mM dichloroacetate (DCA), a chemical inhibitor of PDK1 (*P<0.01). Interestingly, the cell viability of the parental lines does not appear to decrease with treatment of either inhibitor and Aβ. Cell viability was determined by the reduction of the tetrazolium salt MTT. Data are representative of three separate experiments. Data was analyzed by a one-way ANOVA followed by a Tukey test.