Nocodazole Treatment Decreases Expression of Pluripotency Markers Nanog and Oct4 in Human Embryonic Stem Cells
Figure 11
Expression of Nanog and Oct4 in apoptotic cells of hESC treated with nocodazole.
hESC cells were treated with nocodazole as described in Figure 3. Cells were fixed and stained with anti-Nanog (PE), anti-Oct4 (Alexa Fluor 647) and anti-cleaved caspase-3 (detected with anti-rabbit IgG conjugated with Alexa 488) antibodies. (A) Expression of Nanog and (B) Oct4 in cleaved-caspase-3-positive cells. For analysis cellular debris and doublets were excluded. Results are shown as contour plots and are representative of two independent experiments.