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Effects of Solution Chemistry and Aging Time on Prion Protein Adsorption and Replication of Soil-Bound Prions

Figure 1

Solution Chemistry Does Not Affect Short-Term HY TME PrP^Sc Adsorption to Bentonite Clay and Silicon Dioxide Powder.

(A): Representative Western blots of PrP^Sc adsorbed to bentonite clay or silicon dioxide powder (SiO₂) in DPBS, CalCl₂, or NaCl solutions for 1 h, 1 d, or 7 d at 22°C. ‘Control’ indicates 250 µg eq clarified HY TME brain control (DPBS for lane 1, H₂O for lanes 5 and 9). (B): Approximate PrP^Sc recovery in soil pellets (‘Bound’) and supernatants (‘Unbound’). Error bars represent ±1 standard error of the mean.

doi: https://doi.org/10.1371/journal.pone.0018752.g001