Local De Novo Assembly of RAD Paired-End Contigs Using Short Sequencing Reads
Figure 5
Long-insert RAD paired-end contigs.
Increasing the length of RAD fragments isolated before paired-end library preparation (A) and adding two circularization steps (B, C) creates short fragments with two ends that were originally distantly separated. (D) An example of the pileup of reads (grey bars) and the resulting contigs (blue and green lines) from both sides of one SbfI site (short red bars) in E. coli assembled from a long-insert RAD library. The individually-assembled contigs produced were up to 5 kb in length with an N50 length of 3,807 base pairs (E).