Ribosome-Dependent ATPase Interacts with Conserved Membrane Protein in Escherichia coli to Modulate Protein Synthesis and Oxidative Phosphorylation
Figure 2
Localization of RbbA and YhjD on membranes.
(A) Immunoblot analysis of the indicated His6-RbbA fusion protein in whole cell lysates (WCL, panel II) and anti-FLAG immunoprecipitates (IP, panel II) from an untagged DY330 strain and E. coli expressing a SPA-tagged MsbA, LptB and LptD proteins. Expression of affinity-tagged proteins from MsbA, LptB and LptD in cell lysates (panel I) is monitored using M2 anti-FLAG antibody. The unrelated SPA-tagged YchM strain served as control. Molecular masses are shown in kDa. (B) Intracellular localization of RbbA-YFP and YhjD-YFP using MinD-YFP fusion as control. Arrows show the YFP localization at the cell poles (top panel). Percentage YFP cells in one or two ends of the cell poles (bottom panel) for the indicated YFP-strain is an average of at least four experiments. Error bars indicate mean ± SD. Scale bar equals 2 µm. (C) Differential interference contrast images of temperature induced changes of cell morphology and the average cell length of the rbbA-yhjD double mutant and their respective single mutants at indicated temperatures are shown on the right side of panel C. The minDΔ served as control. Scale bar equals 2 µm.