Serum Response Factor Regulates Immediate Early Host Gene Expression in Toxoplasma gondii-Infected Host Cells
Figure 3
Toxoplasma Activation of SRF is Dependent on Rhoptry Secretion.
A.) SRE-CD8 COS cells were mock treated, infected with RH GFP, or treated with 1 µM cytochalasin D. Cells were collected 18 h later and analyzed by flow cytometry after staining with anti-CD8 antibody. Shown are representative results from three independent experiments. B.) SRE-luc-transfected MEFs were mock- infected or infected with untreated parasites (−4BPB) or parasites pretreated with 4-BPB (Toxo+4BPB). In addition, SRE-luc-transfected cells were pretreated with 4-BPB and then infected with untreated parasites (Host+4BPB). Luciferase activity was measured 18 h later. Shown are the averages and standard deviations of three independent experiments performed in triplicate.