Duck Egg-Drop Syndrome Caused by BYD Virus, a New Tembusu-Related Flavivirus
Figure 4
CPE in BYDV infected DEFs and electron micrographs of virus particles.
HE-stained, non-infected DEF control (A); HE stained infected cells, 52 hrs post-infection, showing cell disruption with a large number of red-stained particles (B); Non-infected Vero cell monolayer (C); Infected Vero cells rounded up and focal detachment 60 hrs post-infection (D); Thin section of the infected cell, 24 hrs post-infection, showing dense particles (white arrow head) and virions (arrow) within the vesicles in the cytoplasm (N, nucleus). Insets are magnified virions (E); Negative staining of the purified BYDV from the cell culture supernatants (arrow indicates the typical enveloped flavivirus) (F).