Brahma Is Required for Proper Expression of the Floral Repressor FLC in Arabidopsis
Figure 3
BRM controls expression of FLC.
A) Percentage of flowering under SD (8∶16) conditions of wild-type (Col), brm-1, brm-2, flc-3 and brm-1flc-3 plants. B) Analysis of FLC expression in wild-type (Col) and brm-1 plants by qRT-PCR. Total RNA was isolated from seedlings collected 10 h after dawn at 14 and 15 days of growth under LD conditions and at 19 and 20 days under SD conditions. C) Analysis of the levels of H3K4me3 and H3K27me3 by ChIP-PCR at the FLC promoter in WT and brm-1 mutant plants. A representative experiment of three independent replicates is shown.