Llama-Derived Single Domain Antibodies to Build Multivalent, Superpotent and Broadened Neutralizing Anti-Viral Molecules
Figure 3
Neutralization and fetuin competition of H5N1-specific VHH.
Monovalent VHH neutralizing Influenza H5N1, A/Vietnam/1203/04 in a pseudotyped neutralization assay. Neutralization (geometric mean of duplicates) is expressed in percentage compared to the virus only and plotted against the concentrations of VHH in molarity (M) (A). Dilutions of monovalent VHH against Influenza H5 in duplicates, competing with 10 µg/ml immobilized fetuin for binding to biotinylated H5, expressed as percentage competition. Plotted against concentrations of VHH in molarity (M). NR is an irrelevant VHH binding to RSV. Detection of biotinylated H5 as indicated in text. Standard deviation (SD) indicated by bars (B).